Knowledge equivalent to that which would normally be acquired through experience working in a biological research laboratory setting for a minimum of three years, including demonstrated instrumentation operation and problem-solving skills experience operating flow cytometer(s) and using post-acquisition data analysis software including FlowJo or FCS Express is desirable flow cytometric cell sorting experience is desirable, as well experience in imaging with fluorescence or confocal microscopy would also be advantageous knowledge equivalent to that which would normally be acquired through experience working in a biological research laboratory setting for a minimum of three years, including demonstrated instrumentation operation and problem-solving skills demonstrated attention to detail and ability to adhere to a protocol or standard operating procedure strong verbal and written interpersonal communication skills in daily interactions with Core customers, trainees, service providers, and vendors and ability to work both independently and as part of a team with both Core staff and trainees/customers to tackle technical and administrative challenges. However please note that the facility staff won´t be able to offer assistance on using and coding free software.Knowledge equivalent to that which would be acquired by completing a four-year college degree program such as a BS in Biological Sciences or three to five years of related and progressively more responsible or expansive work experience in overseeing research projects or laboratory operations, operating a variety of laboratory equipment, and experience with cell culture and/or animal research. The Purdue University Cytometry Laboratories has a comprehensive list. Many of these free softwares allow you to write tools that are specific for your analysis. Users will need to create an account on DeNovo Software first and let us know their sign-in account via email, to activate the license into their accounts before they book the workstation.įree flow cytometry software packages are another option if you have experience in coding. ![]() The FCS Express workstation can be booked via PPMS (link at the bottom of this page) and is located in the Sir Alexander Fleming Building, 5th floor, Room 532. We strongly recommend that you watch the SpectroFlo tutorials on migrating your experiment instance before analysing your data.Īn FCS Express license is also available at no extra cost for the Aurora users to analyse raw full spectral cytometry data and, importantly, to overlay the spectra from your single stains for QC purposes (antibody titration, spectra comparison over time and intensity on beads vs cells). The SpectroFlo workstation can be booked via PPMS (link at the bottom of this page) and is located in the Sir Alexander Fleming Building, 5th floor, Room 532. The facility has a powerful computer with SpectroFlo installed that can be used as part of our services at no extra cost. Users will need to download the FlowJo software, and we strongly recommend that you read the FlowJo tutorials and watch the FlowJo webinars before attempting any work.Īurora users are strongly advised to analyse large quantities of data using SpectroFlo, the software specific for Cytek Aurora. Licenses are activated for one week upon request for remote work. Registered users at the facility have access to FlowJo as part of our services at no extra cost.
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